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serum replacement medium (srm  (Thermo Fisher)


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    Thermo Fisher serum replacement medium (srm
    Serum Replacement Medium (Srm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/serum+replacement+medium+(srm/bio_rxiv__2024__10__07__616997-119-21-37
    Average 90 stars, based on 1 article reviews
    serum replacement medium (srm - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Large-Scale Generation and Characterization of Homogeneous Populations of Migratory Cortical Interneurons from Human Pluripotent Stem Cells.
    Article Snippet: MGE differentiation was initiated by passaging H9 cells as spheres in low adherent flasks with SRM media (DMEM with 15% knockout serum replacement [KSR], 2 mM L-glutamine and 10 mM b-mercaptoethanol [all from Thermo Fisher, Waltham, MA, USA]).

    Article Title: LRRK2-mutant microglia trigger dopaminergic neurodegeneration when activated by neuromelanin
    Article Snippet: Generation of vmDAn from iPSCs was performed following the floor plate (FP) derivation protocol , . iPSC medium was changed to Serum Replacement Medium (SRM), which was based on KO-DMEM with 15% of KO Serum Replacement (KO-SR; GibcoTM), 1 % of P/S, 1% of Glut, 1% of Non-Essential Amino Acids (NEAA; Lonza), and 10 μM of β-Mercaptoethanol (BME; GibcoTM).

    Article Title: Large-Scale Generation and Characterization of Homogeneous Populations of Migratory Cortical Interneurons from Human Pluripotent Stem Cells
    Article Snippet: MGE differentiation was initiated by passaging H9 cells as spheres in low adherent flasks with SRM media (DMEM with 15% knockout serum replacement [KSR], 2 mM L-glutamine and 10 μM β-mercaptoethanol [all from Thermo Fisher, Waltham, MA, USA]).

    Targeted Proteomics:

    Article Title: TGF?1 Inhibition Increases the Radiosensitivity of Breast Cancer Cells In Vitro and Promotes Tumor Control by Radiation In Vivo
    Article Snippet: Cell culture Human breast cancer cell lines MCF10A MCF7, MDA-MB-231, T47D and Hs578T cells, and murine breast cancer cell line 4T1 were obtained from American Type Culture Collection and were cultured in MEGM, 10% FBS-MEME, 10% FBS-DMEM, 10% FBS-RPMI, 10% FBS-DMEM, and 10% FBS-DMEM media, respectively, at 37°C with 5% CO 2 . .. Cells were treated in 10% serum replacement medium (SRM; Knockout SR, Life Technologies, Inc.) containing either 500 pg/mL TGFβ (R&D Systems), 400 nmol/L small molecule inhibitor of the TGFβ type I receptor kinase, LY364947 ([3-(Pyridin-2-yl)-4-(4-quinonyl)]-1H-pyrazole); Lilly designation HTS466284; Catalogue no. 616451, Calbiochem) or 1D11, a pan-isoform, neutralizing TGFβ monoclonal antibody or 13C4, murine monoclonal isotype control antibody (Genzyme). ..

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100 nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 7 and 8, 50% SRM with 50% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTM media, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20 ng/ml BDNF, 20 ng/ml GDNF, 0.5 mM dibutyryl cAMP, 0.2 mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype
    Article Snippet: Cells were treated with 10ng/ml of rTGFβ (Life Technologies, cat#: PHG9204) for the indicated times. .. To assess the activity of TGFβ produced by ThPOK kd cells, the cells were grown in RPMI/DMEM with 10% of Serum Replacement Medium (KO SRM Gibco cat# 10828-028) for 72h before the supernatant was collected, centrifuged and filtered to constitute the conditioned media (CM). shScr cells were grown in the presence of conditioned media with or without the TGBRI inhibitor LY2157299, at a 2nM concentration for 24h. ..

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor.
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRMmedia with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μMPurmorphamine, and 3 μMCHIR99021; on days 7 and 8, 50% SRMwith 50% N2media supplemented with 100nM LDN193189 and 3 μMCHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTMmedia, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20ng/ml BDNF, 20 ng/ml GDNF, Nature Communications | (2023) 14:6773 13 0.5mM dibutyryl cAMP, 0.2mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Knock-Out:

    Article Title: TGF?1 Inhibition Increases the Radiosensitivity of Breast Cancer Cells In Vitro and Promotes Tumor Control by Radiation In Vivo
    Article Snippet: Cell culture Human breast cancer cell lines MCF10A MCF7, MDA-MB-231, T47D and Hs578T cells, and murine breast cancer cell line 4T1 were obtained from American Type Culture Collection and were cultured in MEGM, 10% FBS-MEME, 10% FBS-DMEM, 10% FBS-RPMI, 10% FBS-DMEM, and 10% FBS-DMEM media, respectively, at 37°C with 5% CO 2 . .. Cells were treated in 10% serum replacement medium (SRM; Knockout SR, Life Technologies, Inc.) containing either 500 pg/mL TGFβ (R&D Systems), 400 nmol/L small molecule inhibitor of the TGFβ type I receptor kinase, LY364947 ([3-(Pyridin-2-yl)-4-(4-quinonyl)]-1H-pyrazole); Lilly designation HTS466284; Catalogue no. 616451, Calbiochem) or 1D11, a pan-isoform, neutralizing TGFβ monoclonal antibody or 13C4, murine monoclonal isotype control antibody (Genzyme). ..

    Control:

    Article Title: TGF?1 Inhibition Increases the Radiosensitivity of Breast Cancer Cells In Vitro and Promotes Tumor Control by Radiation In Vivo
    Article Snippet: Cell culture Human breast cancer cell lines MCF10A MCF7, MDA-MB-231, T47D and Hs578T cells, and murine breast cancer cell line 4T1 were obtained from American Type Culture Collection and were cultured in MEGM, 10% FBS-MEME, 10% FBS-DMEM, 10% FBS-RPMI, 10% FBS-DMEM, and 10% FBS-DMEM media, respectively, at 37°C with 5% CO 2 . .. Cells were treated in 10% serum replacement medium (SRM; Knockout SR, Life Technologies, Inc.) containing either 500 pg/mL TGFβ (R&D Systems), 400 nmol/L small molecule inhibitor of the TGFβ type I receptor kinase, LY364947 ([3-(Pyridin-2-yl)-4-(4-quinonyl)]-1H-pyrazole); Lilly designation HTS466284; Catalogue no. 616451, Calbiochem) or 1D11, a pan-isoform, neutralizing TGFβ monoclonal antibody or 13C4, murine monoclonal isotype control antibody (Genzyme). ..

    Cell Culture:

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100 nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 7 and 8, 50% SRM with 50% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100 nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTM media, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20 ng/ml BDNF, 20 ng/ml GDNF, 0.5 mM dibutyryl cAMP, 0.2 mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Article Title: Visualization of accessible cholesterol using a GRAM domain-based biosensor.
    Article Snippet: .. On initiation day 0, iPSC media was aspirated, and cells were cultured in SRM medium (KnockOutTM-DMEM/F12, 15% KnockOutTM Serum Replacement, 1% GlutaMAXTM, 1% MEM-Non-Essential Amino Acids Solution, 1% Sodium Bicarbonate 7.5% Solution, 0.1% 2-Mercaptoethanol) (Gibco) containing 100nM LDN193189 and 10 μM SB431542. .. Daily changes of culture media were performed according to the following media composition: on days 1 and 2, cells were cultured in SRM media with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), and 2 μM Purmorphamine; on days 3 and 4, SRMmedia with 100 nM LDN193189, 10 μM SB431542, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μM Purmorphamine, and 3 μM CHIR99021; on days 5 and 6, 75% SRM media with 25% N2 media (NeurobasalTM media, 1% N-2 Supplement, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) with 100 nM LDN193189, 100 ng/ml recombinant mouse sonic hedgehog/Shh (C25II), 2 μMPurmorphamine, and 3 μMCHIR99021; on days 7 and 8, 50% SRMwith 50% N2media supplemented with 100nM LDN193189 and 3 μMCHIR99021; on days 9 and 10, 25% SRM with 75% N2 media supplemented with 100nM LDN193189 and 3 μM CHIR99021; on days 11 and 12, NB/B27 media (NeurobasalTMmedia, 2% B-27TM Supplement without vitamin A, 1% GlutaMAXTM) (Gibco) supplemented with 3 μM CHIR99021, 20ng/ml BDNF, 20 ng/ml GDNF, Nature Communications | (2023) 14:6773 13 0.5mM dibutyryl cAMP, 0.2mM ascorbic acid, 1 ng/ml TGF-β3, and 10 nM DAPT.

    Activity Assay:

    Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype
    Article Snippet: Cells were treated with 10ng/ml of rTGFβ (Life Technologies, cat#: PHG9204) for the indicated times. .. To assess the activity of TGFβ produced by ThPOK kd cells, the cells were grown in RPMI/DMEM with 10% of Serum Replacement Medium (KO SRM Gibco cat# 10828-028) for 72h before the supernatant was collected, centrifuged and filtered to constitute the conditioned media (CM). shScr cells were grown in the presence of conditioned media with or without the TGBRI inhibitor LY2157299, at a 2nM concentration for 24h. ..

    Produced:

    Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype
    Article Snippet: Cells were treated with 10ng/ml of rTGFβ (Life Technologies, cat#: PHG9204) for the indicated times. .. To assess the activity of TGFβ produced by ThPOK kd cells, the cells were grown in RPMI/DMEM with 10% of Serum Replacement Medium (KO SRM Gibco cat# 10828-028) for 72h before the supernatant was collected, centrifuged and filtered to constitute the conditioned media (CM). shScr cells were grown in the presence of conditioned media with or without the TGBRI inhibitor LY2157299, at a 2nM concentration for 24h. ..

    Concentration Assay:

    Article Title: Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4 + cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotype
    Article Snippet: Cells were treated with 10ng/ml of rTGFβ (Life Technologies, cat#: PHG9204) for the indicated times. .. To assess the activity of TGFβ produced by ThPOK kd cells, the cells were grown in RPMI/DMEM with 10% of Serum Replacement Medium (KO SRM Gibco cat# 10828-028) for 72h before the supernatant was collected, centrifuged and filtered to constitute the conditioned media (CM). shScr cells were grown in the presence of conditioned media with or without the TGBRI inhibitor LY2157299, at a 2nM concentration for 24h. ..



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